Here, you calculate the doubling time of your cells online. It's helpful to manage cell culture.
The doubling time equation works for any kind of exponentially growing object.
I didn't mention any unit for time and concentrations. It's not necessary.
Keep in mind that the doubling time unit is the same than the one you used for the duration.
Instead of using concentration of cells, you can use the number of cells.
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Two Time Points |
Several Time Points
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EXAMPLE:
Doubling Time = 21.86=ln(2)/0.0317
Growth Rate = 0.0317
Growth Rate = number of doublings that occur per unit of time
Equation : amount=0.5288*e0.0317*time
At t=0, calculated cell concentration = 0.5288
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Web site cited in Pubmed:
- In vitro cytotoxicity of novel platinum-based drugs and dichloroacetate against lung carcinoid cell lines. Fiebiger W et al., Clin Transl Oncol. 2011
- Transcriptional silencing functions of the yeast protein Orc1/Sir3 subfunctionalized after gene duplication. Hickman MA., Rusche LN., PNAS 2010
- Mesenchymal stem cells or cardiac progenitors for cardiac repair? A comparative study. Koninckx R. et al, Cellular and Molecular Life Sciences 2010
- Detailed Molecular Fingerprinting of Four New Anaplastic Thyroid Carcinoma Cell Lines and Their Use for Verification of RhoB as a Molecular Therapeutic Target. Marlow LA. et al, Journal of Clinical Endocrinology & Metabolism 2010
- Outgrowth of fibroblast cells from goat skin explants in three different culture media and the establishment of cell lines. Singh M., Sharma AK., In Vitro Cell.Dev.Biol.—Animal 2010
- Abrogation of E-Cadherin-Mediated Cellular Aggregation Allows Proliferation of Pluripotent Mouse Embryonic Stem Cells in Shake Flask Bioreactors. Mohamet L. et al, PLoS One 2010
- Serine 649 Phosphorylation within the Protein Kinase C-Regulated Domain Down-Regulates CARMA1 Activity in Lymphocytes. Moreno-García E. et al, The Journal of Immunology 2009
- Adult Palatum as a Novel Source of Neural Crest-Related Stem Cells. Widera D. et al, Stem cells 2009
Cite this website as following:
Roth V. 2006 <http://www.doubling-time.com/compute.php>
