Doubling Time
Tired of overcrowding ?

Not enough cells to
start the experiment ??
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Here, you calculate the doubling time of your cells online. It's helpful to manage cell culture.
The doubling time equation works for any kind of exponentially growing object.
I didn't mention any unit for time and concentrations. It's not necessary.
Keep in mind that the doubling time unit is the same than the one you used for the duration.
Instead of using concentration of cells, you can use the number of cells.
Two Time Points
Several Time Points
Initial concentration :
Final concentration :
Duration of culture :
 
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Paste your data here
(couple time-amount):

What is the 1st column ?
Time
Amount

 EXAMPLE:
Doubling Time = 21.86=ln(2)/0.0317
Growth Rate = 0.0317
Growth Rate = number of doublings that occur per unit of time
Equation : amount=0.5288*e0.0317*time
At t=0, calculated cell concentration = 0.5288
Exponential Curve Fitting
Web site cited in Pubmed:
Adult Palatum as a Novel Source of Neural Crest-Related Stem Cells. Widera et al, Stem cells 2009

Cite this website as following
Roth V. 2006 <http://www.doubling-time.com/compute.php>

Reference for exponential regression:
Weisstein, Eric W. "Least Squares Fitting--Exponential." From MathWorld--A Wolfram Web Resource. http://mathworld.wolfram.com/LeastSquaresFittingExponential.html
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